ABSTRACT

Chronic obstructive pulmonary disease (COPD) is the major cause of mortality rate all over the globe. An irreversible decline in lungs function leads to permanent alteration in surface area of alveoli. COPD decreases the immune of the patient leading to asthma, chronic bronchitis and other pulmonary diseases. The pathogenic organisms were isolated from the remnant sputum samples of COPD patients. Based on the phenotypic characteristics isolates suspected to be Klebsiella pneumonia were selected and tested for biochemical characterization using the pure colony. Up on performing Antibiotic susceptibility test using various antibiotics by standard Kirby Bauer method, all the isolated were found to be highly sensitive to chloramphenicol, than other antibiotics such as Meropenem, imipenem, ertapenem gentamycin, Co-trimoxazole, cefotaxime. To study the genetics and to identify the isolates at genetic level, molecular studies were performed through fingerprinting which makes easy identification. DNA was extracted and gel Electrophoresis technique was carried to check the quality of the DNA isolated. RAPD was performed at an appropriate annealing temperature. Upon performing gel electrophoresis the amplified products were observed, amongst which 4 samples appear similar which can be classified as a same subtype. And these obtained bands were compared which may aid to prepare a dendrogram of Klebsiella spp. AIMS: The purpose of this study is to investigate, isolate the bacterial pathogens and study the microbiology of disease to increase our understanding about the pathogenesis and other consequences in COPD. Keywords: Chronic Obstructive Pulmonary Disease, Klebsiella pneumonia, antibiotic susceptibility test, RAPD
Publication date: 01/11/2021

https://ijbpas.com/pdf/2021/November/MS_IJBPAS_2021_5703.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.11.5703