ABSTRACT

Cosmetics are personal care products which containing water and organic / inorganic compounds that are sensitive to microbes, due to the possibility of cross contaminations during manufacturing, filling, and packing process. Hence, it required the right preservation against microbial contamination to ensure the safety and shelf life of the product. The most common strategies are the application of anti-microbial agent, either by using Natural or synthetic compounds including multifunctional ingredients. Therefore, the present study is to evaluate the preservative effect of the methanolic leaf extract of Acalypha indica L., collected from Pallipattu village of Thiruvallur district of Tamil Nadu, India. In Cosmetic preparation by invitro study, the crude methanolic extract re-dissolved in propylene glycol was examined for Preservative effect (challenge and re-challenge test) by In-vitro method followed the method of Cosmetic, Toiletry, and Fragrance Association 2007. (CTFA) Microbiology Guidelines. The anti-microbial effect of Acalypha indica L., effect of 1.00 to 2.00% of concentrations tested against the gram-negative bacteria Pseudomonas aeruginosa and the fungal species Aspergillus niger by using Agar plate count method at various intervals up to 28 days. The result of the study indicates that decreasing of microbial cells at increasing intervals such as 24hrs, 48hrs, 5days, 7days, 14days, 21days and 28days. However, after 5 days of incubation, the microbial growth is completely inhibited as nil growth observed even up to 28days period. The present study concludes that the compounds extracted from Acalypha indica L has moderate to significant level of antimicrobial effect against the microbes in the cosmetic preparations which could be used as a good natural preservative in various personal care products especially cosmetic preparations. Key words: Cosmetics preparation, Antimicrobial activity, Acalypha indica L, Preservatives
Publication date: 01/10/2021

https://ijbpas.com/pdf/2021/October/MS_IJBPAS_2021_OCT_SPCL_1044.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.10.1044