ABSTRACT

Fifty one fungi were isolated from eight soil samples collected from distinct habitats of Kalaburagi region and screened for their antimicrobial activity against nine Candida spp. and five bacterial strains. Twenty isolates were picked up by primary screening using perpendicular streak plate method and subjected to secondary screening by agar well diffusion assay. Among the fungal isolates, two with code VSGF1 and VSGF2 have shown significant zone of inhibition against all tested pathogenic microbes. The crude ethyl acetate extract of VSGF1 displayed significant zone of inhibition 16.67±0.33mm and 15.00±0.57mm against Candida glabrata (MTCC3981), C. albicans (MTCC1637) and C. tropicalis (MTCC1406), respectively. However, the VSGF2 exhibited highest of 15.00±0.57mm inhibition zone against Escherichia coli and 14.33±0.33mm against Bacillus subtilis and Staphylococcus aureus. Mycochemical screening of both fungal isolates showed the presence of alkaloids, flavonoids, phenols, saponins and tannins, with highest contents of flavonoids followed by alkaloids and phenols. The molecular identification of VSGF1 and VSGF2 was done and confirmed their identity as Talaromyces islandicus and Aspergillus terreus, respectively. The efficient antimicrobial activity of both the fungi shall be considered while using the fungi as potential source for new antimicrobial drug. Keywords: Antimicrobial activity, Candida spp., Mycochemical screening, Molecular identification, Soil fungi
Publication date: 01/10/2021

https://ijbpas.com/pdf/2021/October/MS_IJBPAS_2021_OCT_SPCL_1031.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.10.1031