ABSTRACT

Two plant species of the genus Vigna were selected for the study. These are Vigna mungo L Hepper and Vigna radiata L. R. Wilczek. Both were authenticated and identified from the BSI, Pune. Seeds of both plants were selected for the analysis. Plant material was extracted by using ethanol and water as a solvent. A column chromatographic technique was used for the separation of the compounds from the extract by using n-hexane, chloroform and methanol in different concentrations. Total four fractions were selected for the study. Those are VMF1, VMF2, VRF3 and VRF4. Preliminary phytochemical tests were performed. EE of Vigna mungo was showing presence of carbohydrates, saponins, flavonoids, alkaloids and tannins in preliminary analysis. Carbohydrates, saponins, flavonoids, phenolics and tannins were identified in the EE of Vigna radiata. VMF1 and VMF2 was indicating presence of the flavonoids and phenolic compounds in significant concentrations. VRF3 and VRF4 of Vigna radiata were indicating the flavonoid and phenolic compounds. Phenolic acids and flavonoids quantification of fractions isolated from na mungo and Vigna radiata was performed by using 10 standards by using HPLC-DADMS/MS system. Extracted ion chromatogram of Vigna mungo VMF1 shows presence of rutin and quercetin.VM fraction 2 shows rutin and quercetin. Gallic acid, syringic acid, quercetin and kaempferol are present in Vigna radiata fraction 3. Syringic acid was found to be present in higher concentration. Fraction 4 of Vigna radiata shows presence of syringic acid, cinnamic acid and quercetin. Keywords: Phenolic Acids and Flavonoids Quantification, Vigna Mungo, Vigna Radiata
Publication date: 01/10/2021

https://ijbpas.com/pdf/2021/October/MS_IJBPAS_2021_OCT_SPCL_1026.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.10.1026