ABSTRACT

A Specific, Precise, Accurate, Linear and Robust method was developed for the simultaneous estimation of the Cobicistat and Atazanavir in tablet dosage form and Validated done as per ICH Validation guidelines. Method was optimized by Zorbax CyanoColumn (150*4.6mm &5?m) column at a flow rate of 1.0ml/min, Mobile phase was 0.1% Orthophosphoricacid: Methanol (75:25). The Column Oven temperature was maintained at 25°C and working wave length was selected at 234nm. The retention times of Cobicistat and Atazanavir were found to be 2.853min and 4.090min respectively. % RSD of the Cobicistat and Atazanavir were and found to be 0.48% and 0.62% respectively. In Method precision Parameter, % Assay was found 95.0 to 105.0% and % Recovery were obtained as 99.8% and 100.3% for Cobicistat and Atazanavir respectively. Linearity was obtained as 0.9996 and 0.9995 for Cobicistat and Atazanavir.Analytical Range was found from the linearity and accuracy for Azelnidipine was 4?g/mL to 12?g/mL and Telmisartan was 40?g/mL to 80?g/mL. Keywords: Cobicistat and Atazanavir and Zorbax CN Column
Publication date: 25/09/2021

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https://doi.org/10.31032/IJBPAS/2021/10.9.1018