ABSTRACT

Oleanolic acid is an important pentacyclictriterpenoid saponin exists widely in plants in the form of free acid (or) aglycone. Oleanolic acid is traditionally used in Asian medicine as anti-inflammatory, anti-hyperlipidemic and hepatoprotective substance. Triterpene precursors such as squalene, Farnesyl pyrophosphate (FPP), Isopentenyl pyrophosphate (IPP) are involved in the triterpene biosynthetic pathway. In the present study, the effect of squalene, IPP, FPP (1-5 mg/l) were studied on OA production in Lantana camara callus cultures, developed using root explant. Oleanolic acid content in squalene treated callus was determined by RPHPLC. From the resultsit was evident that squalene (3 mg/l) was found to be more effective on oleanolic acid production in callus cultures of Lantana camara compared to control callus. Squalene precursor fed callus also produced the highest biomass compared to the callus treated with the other precursors. Keywords: Lantana camara, precursors, callus cultures, Oleanolic acid

https://ijbpas.com/pdf/2021/September/MS_IJBPAS_2021_SEPT_SPCL_1013.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.9.1013