ABSTRACT

Urolithiasis is the presence of calculi in the kidney and is also found in the urinary tract, the ureters and bladder. The urinary stone formation known as urolithiasis is a serious attenuate problem throughout the world. Struvite is a triple phosphate stone found more frequently in women. Urinary stones are characterized by high repetition rate therefore requiring a preventive treatment by using the medicinal plants. Alpinia zerumbet Pers. commonly known as Shell Ginger belongs to the family Zingiberaceae. Medicinal uses of Alpinia zerumbet contains anti-pyretic, anti-depressant, analgesic, anti-inflammatory, anti-cancer and anti-ulcer activities. The inhibitory effect of ethanol extract ofAlpinia zerumbet rhizome on the growth of struvite crystals were investigated. Struvite crystals have been grown by the single diffusion gel growth technique. The weight of the formed struvite crystals were gradually reduced from 2.36 g to 0.30 g with increase in the concentration of ethanol extract of Alpinia zerumbet rhizome. Presence of bioactive compounds like Tannin, Phlobatannins, Alkaloids, Flavonoids, Saponins, Phenols, Steroids, Carbohydrates, Xanthoprotein, Leucoanthocyanin, Anthoquinines, Emodine, Terepenoids and Anthocyanins was confirmed by Preliminary phytochemical analysis of the rhizome extract. The major bioactive compounds of Alkaloid, Flavonoid and Terpenoid are present. The struvite crystals have been harvested and characterized by Fourier Transform Infrared Spectroscopy (FTIR) to confirm the functional groups. The FTIR spectrum of harvested struvite crystals from various treatments showed shifts in bands, disappearance of existing and appearance of new peaks which indicated the activity of bioactive components in the extract. This study confirms that using ethanol extract of rhizomes of Alpinia zerumbet rhizome can promote the formation of ammonium magnesium phosphate hexahydrate crystals and reduce the nucleation rate of struvite crystal is a main component of triple phosphate urinary stone. Keywords: Urolithiasis, Struvite crystals, Alpinia zerumbet, Fourier Transform Infrared Spectroscopy
Publication date: 01/10/2021

https://ijbpas.com/pdf/2021/October/MS_IJBPAS_2021_5656.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.10.5656