ABSTRACT

Total six bacterial isolates (SL1, SL2, SL3, ST1, ST2 and ST3) were obtained from soil sample collected from two different local textile industries, Solapur. On the basis of qualitative screening for dye decolorization ST3 was found to decolorize nine structurally different textile dyes compared to other five (Sl1, SL2, SL3, ST1 and ST2) isolates hence it was selected for further studies. On the basis of % dye decolorization isolate ST3 (75%) found to be more effective in removing DNB (Direct Navy Blue) dye as compared to other dyes within 24h. Isolate ST3 showed maximum dye decolorization activity at Temperature (30±2oC), pH (7), inoculum size (8% v/v), dye concentration (20ppm), 1%(w/v) beef extract and nutrient broth medium whereas decolorization was not much influenced by static and shaking conditions. The isolate showed a positive correlation between growth and dye decolorization; hence it is biodegradation and not mere cell adsorption. The bacterial strain ST3 was identified by rDNA sequencing as Shewanella decolorationis. Keywords: Screening, decolorization, dye Direct Navy Blue, optimum, Shewanella decolorationis
Publication date: 01/10/2021

https://ijbpas.com/pdf/2021/October/MS_IJBPAS_2021_5653.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.10.5653