ABSTRACT

The green synthesis of nanoparticles from plant extract has been increasingly gaining popularity due to its eco-friendly nature and cost-effectiveness. In this study, Silver nanoparticles were synthesized using the fruit peel extract of Citrus maxima. The synthesized AgNPs characteristics were confirmed by using several studies includes UV-Vis spectroscopy, SEM, XRD and FTIR. The results obtained from the UV–visible spectroscopic analysis showed that the intense surface plasmon resonance band at 425 nm which indicates the formation of AgNPs. The morphology of AgNPs was observed under scanning electron microscopy displayed that the synthesized AgNPs have a spherical shape with an average size ranges from 38-65 nm in diameter. The XRD study showed that the particles were crystalline in nature, with FCC structure. FTIR indicated that the AgNPs capped with active ingredients of the fruit peel extract. The performance of fruit peel extract of Citrus maxima AgNPs in terms of inhibition of bacteria growth was studied by disc diffusion. Synthesized fruit peel extract of Citrus maxima AgNPs were found to possess enhanced antibacterial properties. The nanoparticles were assessed for their cytotoxic activity on HeLa (cervical cancer) cell line. MTT assay is colorimetric assay used for measure the cytotoxicity, thesilver nanoparticles to inhibit the cancer cell line. The HeLa cells treated with AgNPs induce the apoptosis through DNA damage was confirmed in DNA fragmentation assay. Keywords: Citrus maxima, fruit peel, characterization, cytotoxicity, MTT, HeLA
Publication date: 01/10/2021

https://ijbpas.com/pdf/2021/October/MS_IJBPAS_2021_5648.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.10.5648