ABSTRACT

The present article portrays simple, sensitive, accurate, precise and cost effective First order derivative Spectrophotometric method and HPTLC method for the simultaneous estimation of Telmisartan and Nebivolol in bulk and combined dosage form. In The first order derivative method absorption at 283 nm (zero crossing point for Nebivolol) was used for Telmisartan and 278 nm (zero crossing point for Telmisartan) was used for Nebivolol. The linearity was taken in the concentration range of 10-50 ?g/ml and 5-25 ?g/ml for Telmisartan and Nebivolol respectively. In the HPTLC method, the chromatograms were developed using a mobile phase of Toluene: Ethyl Acetate: Methanol: 25% Ammonia (6: 3: 3: 0.2 v/v/v/v) on precoated plate of 200-?m layers of silica gel 60 F254S and quantified by densitometric absorbance mode at 290 nm. The Rf of Telmisartan and Nebivolol were 0.46 and 0.57 respectively. Proposed technique has been validated as per ICH Guideline and can be used in routine pharmaceutical analysis. Keywords: Telmisartan, Nebivolol, HPTLC, Spectrophotome
Publication date: 01/10/2021

https://ijbpas.com/pdf/2021/October/MS_IJBPAS_2021_5636.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.10.5636