ABSTRACT

The study aimed to establish tissue culture protocols for Datura species. Callus tissues of Datura stramonium and D.innoxia were initiated on Murashige and Skoog (MS) medium supplemented with various combinations of auxins {2,4-dichlorophenoxy acetic acid (2,4-D), indole-3-acetic acid (IAA), ?-naphthalene acetic acid (NAA)}, and cytokinin {6-benzyl amino purine (BAP), Kinetin} were used. Tissue culture was initiated from leaves and sterile seedlings after breaking seed dormancy. Callus culture was established and the most effective growth regulators were Kin, IAA and 2,4-D for D.stramonium leaf explant, The concentration 1mg/l Kin with 0.5mg/l 2,4-D was the best for callus formation. D.innoxia leaf initiated callus on MS medium supplemented with 2mg/l Kin and 0.5-1mg/l 2,4-D. MS medium supplemented with 4mg/l IAA, 1.5mg/l Kin and 0.5mg/l 2,4-D was the best for seed explant of D.stramonium and D.innoxia as well. 2,4-D alone was better for callus maintenance and growth. UV light increased callus mass. Calli from the two species treated with NaCl senescenced and died. There were no remarkable changes on callus treated with GA3. Insertion of platinum wire in callus tissue enhanced callus growth in D. stramonium and D.innoxia, resulting in a compact calli for seeds and leaves. Keyword: Datura Stramonium, Datura innoxia, ultra violet, sodium chloride, Gibberellic Acid, Platinum wire
Publication date: 01/09/2021

https://ijbpas.com/pdf/2021/September/MS_IJBPAS_2021_5633.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.9.5633