ABSTRACT

Phyllanthus amarus is has been reported to possess wide range of pharmacological and therapeutic activities. The present study discloses a simple and improved microemulsion formulation system to enhance solublity and stability of standardised aqueous extract of Phyllanthus amarus [PAAE]. The solublity of Phyllanthus amarus aqueous extract in different oils, surfactant and cosurfactant was determined to find the optimal components for microemulsion. Rose oil was selected as oil phase, while tween 80 and PEG 400 were selected as surfactant and cosurfactant respectively. Based on higher solublity values Pseudoternary phase diagram was constructed in order to find the monodisperse region. The prepared microemulsion was evaluated for pH, viscosity, refractive index globular size, zeta potential, poly dispersity index, drug entrapment, percentage drug loading. The formulated microemulsion shows single phase distribution with excellent stability suitable for producing pharmacological activity by various routes of administration. The validated preclinical acute pain model was used to asses the Anti-hyperalgesic activity of the PAAE microemulsion viz. Carrageenan induced inflammatory hyperalgesia. The results of the present study indicate that the formulated microemulsion can alleviate muscle mediated pain and strongly suggest that this formulation can be useful in treatment of acute musculoskeletal hyperalgesia. Keywords: Phyllanthus amarus, microemulsion, Anti-hyperalgesic
Publication date: 01/09/2021

https://ijbpas.com/pdf/2021/September/MS_IJBPAS_2021_5629.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.9.5629