ABSTRACT

Staphylococcus aureus infection is characterized by tissue damage accompanied by a purulent abscess. Some of the other infectious diseases caused by S. aureus are acne, boils, impetigo and wound infections. More severe infections include pneumonia, mastitis, phlebitis, meningitis, urinary tract infections, osteomyelitis, and endocarditis. S. aureus can also cause nosocomial infections, food poisoning, and toxic shock syndrome. The detection of S. aureus bacteria by conventional means can use the culture method. However, the culture method has several disadvantages including: it requires laboratory infrastructure for biosafty lavel 2, requires experts, takes a long time (up to 1 week) to get positive results. Therefore, the proposed research aims to develop a fast and accurate detection for S.aureus detection using the Polymerase Chain Reaction (PCR) method. This method has been developed in line with the development of the discovery of genes contained in S. aureus bacteria such as mecA. The stages of the research method consisted of S. aureus subcultures, primary analysis, DNA isolation, DNA amplification, electrophoresis, and data analysis. The results showed that a pair of primers could amplify the mecA gene with an amplicon length of 161 bp. The results of the optimization of the PCR components get optimal results by obtaining the appropriate DNA bands. The PCR method developed can be used to detect the mecA gene. Keywords: DNA, agarose gel electrophoresis, mecA gene, PCR, S. aureus
Publication date: 01/09/2021

https://ijbpas.com/pdf/2021/September/MS_IJBPAS_2021_5615.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.9.5615