ABSTRACT

Diabetes mellitus is an endocrine disorder, characterized by hyperglycemia with abnormal carbohydrate, protein and lipid metabolism. Currently available treatments have several limitations such as reduced response with prolonged use and toxic side effects. Hence, herbal sources are considered as an alternative for effective management of diabetes mellitus and its associated complications. Commercial antidiabetic polyherbal formulations are available with claimed hypoglycemic activities; yet they lack systematic studies on mechanism of action. In the present study, hydro-alcoholic and aqueous extracts of commercially available antidiabetic polyherbal formulation, Sugar Nil, were assessed for their effects on glucose adsorption, diffusion, amylolysis kinetics and transport across the membrane of yeast cells using in-vitro techniques. Our results revealed that the adsorption of glucose increased dose dependently (5mM -100mM), highest glucose binding capacity was observed (64.8±0.54 mM and 60.74±0.74mM) at 100mM concentration for hydro-alcoholic and aqueous extracts respectively. Rate of glucose uptake into yeast cells was linear (5mM - 25 mM) for both the extracts, whereas glucose diffusion was directly proportional to the time (30 - 180 min). GRDI was calculated to be 17.00%and 9.68% at 30 min which reduced to 7.87% and 3.54% at 120 min for hydro-alcoholic and aqueous extracts respectively. Thus, the observed mode of actions might produce a concerted role of Sugar Nil for decrease in the rate of glucose absorption, thereby confirming its hypoglycemic activity. Keywords: Diabetes mellitus, Polyherbal formulations, Glucose diffusion, Amylolysis kinetics, Yeast
Publication date: 01/09/2021

https://ijbpas.com/pdf/2021/September/MS_IJBPAS_2021_5596.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.9.5596