ABSTRACT

Background: Aloe Vera gel mainly has antifungal, anti inflammatory property due to which it is in major demand in the herbal area of research. Aloe Vera is clear gel with refractive index of 1.3; hence it was tried as substitute to cedar wood oil. On prolonged use cedar wood oil damages the objective lens. Aim: To evaluate the quality of Aloe vera as immersion medium for microscopy in peripheral smear. Materials And Methods: Aloe vera leaves were collected, filleted, grinded and filtered using micro filter .Blood samples were taken from 10 people and peripheral smear was done on the glass slide. The extracted gel is then used as immersion medium in peripheral smears. Evaluation was done. Morphological alterations, nuclear details, cytoplasm details, background, artifacts, debris, airbubbles, ease of cleaning were compared with Cedar wood oil and Aloe Vera gel in microscope for peripheral smear. Results: The results were tabulated. Student t test was used for comparing the various parameters like morphological alterations, nuclear details, cytoplasmic details, background, air bubbles, artifacts .debris and ease of cleaning. Even though the results were no statistically significant aloe vera showed better cytoplasmic details than cedar wood oil and the average value for ease of cleaning for aloe vera and cedar wood oil is 2, 1 respectively. Keywords: Immersion oil, peripheral blood smear, aloe vera gel, refractive index
Publication date: 01/08/21

https://ijbpas.com/pdf/2021/August/MS_IJBPAS_2021_5564.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.8.5564