ABSTRACT

Background Gomphrena globosa, is an edible plant from the family of Amaranthaceae. The flowers are rich in betacyanin and possess red violet colour. It has a stable pH ranging between 3 to 7. To our knowledge, the current study represents the first initiative of using an extract of this flower in staining of oral tissue sections. Aim This experimental comparative study aimed to explore the efficiency of Gomphrena globosa as natural staining dye for oral tissue histological sections as compared to the routinely and commonly used stain, hematoxylin and eosin. Materials and Methods Both fresh flower petals and dried flower petals were used for the process of preparation of the stain. Four different methods were used for the process of stain preparation. Fresh petals were grounded in mortar and pestle and this filtered and used. Dried flower petals were ground into fine powder. One portion of the fresh extract was boiled to 60°C and then this was then used staining purpose. 15 g of this powder was then mixed in 100 ml of distilled water. This was then filtered and used. Finally the 2.5 kg of fine powder was mixed in 500 ml of distilled water for 48 hours. This was then filtered. This solution was then placed in a Lyophilizer for 15 days at -55°C for freeze drying. The viscous mixture thus obtained was used for the process staining. Results The efficacy of the Globosa stain was nil when compared to routine H&E staining. Conclusion Though the pigment molecules present in Gomphrena globosa (Betacyanins) were same as compared to beetroot, the complexity in its chemical structure did not facilitate ionization, Thus the staining efficacy was nil. Keywords Betacyanins, Gomphrenin, Lyophilizer, Maceration
Publication date: 01/08/21

https://ijbpas.com/pdf/2021/August/MS_IJBPAS_2021_5561.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.8.5561