ABSTRACT

Background: Dental Plaque is a sticky coat that contains tons of bacteria which further leads to periodontal problems. And periodontitis is one of the most common oral disease among the Asian population. Anti-inflammatory, immune modulation, anticancer, antioxidant, anti-glycemic and hepatoprotective activities are some biological activities of Nigella sativa seed crude extract. Aim: The aim of this study was to formulate oil-in-water emulsion of Nigella sativa seed oil extract and to investigate its antimicrobial activity against common the pathogens present in dental plaque so as to prevent periodontal problems. Materials and methods: Samples of dental plaque were collected from 8 patients and Streptococcus and Staphylococcus cultures were grown in Blood Agar & Mannitol Salt Agar. Various biochemical tests were done to determine the bacterial species present in the given sample. Then Antibacterial Activity of Nigella sativa seed oil in water emulsion was done using Micro-Broth Dilution method using Resazurin dye. Result: Antibacterial activity of Nigella sativa as oil in water emulsion was present for staphylococcus species in a Minimum Inhibitory Concentration of 1:5 and 1:6, but for streptococcus the activity was less when compared to staphylococcus. Conclusion: Based on the result of this study, it can be concluded that Nigella sativa seed oil possesses antibacterial activity for staphylococcus species when compared to streptococcus present in dental plaque. Keywords: Nigella sativa, periodontitis, plaque, blood agar, mannitol salt agar, resazurin
Publication date: 01/08/21

https://ijbpas.com/pdf/2021/August/MS_IJBPAS_2021_5557.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.8.5557