ABSTRACT

A simple, accurate and reproducible HPTLC method has been developed for the simultaneous quantification of Gallic acid, Quercetin and Glycyrrhizin in the methanolic extracts of Abrus precatorius (MEAP) and Cordia wallichi leaves (MECW). The method was successfully applied for quantification of three biomarkers in the formulations prepared from methanolic extracts of Abrus precatorius and Cordia wallichi leaves. The CAMAG HPTLC systems with Win CATS;Version 1.4.9 software used for the analysis. Double development with mobile phase toluene: ethyl acetate:formic acid:methanol (4.5: 3: 1: 1.5 v/v/v/v) on TLC plate silica gel 60 F254. Detection and quantification of Gallic acid (GA), Quercetin (QUE) and Glycyrrhizin (GLY) were carried out before derivatization with anisaldehyde sulphuric acid reagent. The quantity of Gallic acid, Quercetin and Glycyrrhizin in MEAP was found to be 0.59%, 0.15%, 0.11% and the in MECW the content of gallic acid and quercetin was found 0.35% and 0.23% respectively. In the Polyherbal floating tablet, the quantity of Gallic acid, Quercetin and Glycyrrhizin equivalents (GAE, QE and GE) were found to be 0.46%, 0.19%, 0.05% respectively. The developed method can be successfully applied for quantification of these three components in any formulation containing Abrus precatorius and Cordia wallichi leaf extract. Keywords: Simultaneous quantification, HPTLC, Gallic acid, Quercetin, Glycyrrhizin, Abrus precatorius, Cordia wallichi
Publication date: 01/03/21

https://ijbpas.com/pdf/2021/March/MS_IJBPAS_2021_54111.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.3.5411