ABSTRACT

Diabetes mellitus is a disease with a component of oxidative stress. Oxidative stress is an imbalance condition between the number of free radical molecules and antioxidant compounds in the body which can cause damage to DNA, protein, and lipid. Damage to lipids is called lipid peroxides where oxidized lipids can trigger the production of toxic aldehydes such as Malondialdehyde and are associated with a state of insulin resistance.This study aims to determine the effect of the ethanol extract of Moringa leaves on constant insulin tolerance test (CITT) values and MDA levels in insulin resistance and insulin deficiency mice.Insulin resistance testing was carried out on a preventive basis for 28 days using a high-fat emulsion 0.5 mL//kgBW induction and a curative insulin deficiency test using 50 mg//kgBW of alloxan monohydrate induction.Each study was divided into 6 groups of mice test animals, namely negative control, positive control, control of the ethanol extract of Moringa leaves with a dose of 75 mg//kgBW, 150 mg//kgBW, and 300 mg//kgBW, a comparison control glibenclamide for insulin-deficient animals, and metformin control comparators for insulin resistance comparators. Parameters observed in the insulin resistance method KGD and CITT by giving insulin 0.0125 U//kgB Wintraperitoneal and in the insulin deficiency method, measuring KGD and MDA levels using a UV-Vis spectrometer. The results showed that the effective dose of the method of insulin deficiency, and insulin resistance was 75 mg//kgBW which could affect blood glucose levels, increase insulin sensitivity and reduce malondialdehyde levels in mice. The conclusion of this study indicated that the ethanol extract of Moringa leaves affected the blood glucose levels of the tested animals by increasing the CITTvalue and reducing malondialdehyde levels. Keywords: Antidiabetic, CITT, Malondialdehyde, Moringa oleifera
Publication date: 01/07/21

https://ijbpas.com/pdf/2021/July/MS_IJBPAS_2021_5555.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.7.5555