Azima tetracantha is a plant commonly used as a traditional herbal medicine and
possesses the wide range of pharmacological applications. The main objective is to investigate
the anti-urolithiatic property of various extract of Azima tetracantha on ethylene glycol (EG)
induced urolithiasis (0.75% v/v in drinking water for 28 days) in rats. Male wistar rats were
divided into 7 groups as follows, Group I served as control treated with 4% w/v gum acacia 1.0
mL/kg/ orally for 28 days, Group II treated with ethylene glycol (0.75% v/v) for 28 days, Group
III, IV, V, VI and VII received with cystone, various extracts ofethanolic, ethylacetate,
chloroform and petroleum ether of Azima tetracantha respectively from 15-28th day along with
EG for 28 days. After completion of the 28 days respective treatments, the level of various
urolithiatic promoters in the biological samples such as urine, serum and kidney homogenate.
The rats were sacrificed for isolation of kidney for histopathological evaluation and biochemical
estimation. Biochemical markers like ACP, ALP, AST, ALT and LDH were evaluated in serum
as well as in kidney homogenate. The ethanolic extract of Azima tetracantha showed significant
anti-urolithiatic property when compared with other extracts by increased urine volume,
decreased calcium, phosphate, oxalate levels and increased the magnesium levels in urine significantly (P< 0.001), the serum levels of urea, uric acid, creatinine and BUN were
significantly (P< 0.001) reduced, the biochemical markers were significantly (P < 0.001) reduced
in both serum and kidney homogenate. From the above results it was concluded that Azima
tetracantha having the antiurolithiatic property against ethylene glycol in rats. Hence this plant
may be considered as one of the source for the isolation of new oral anti urolithiatic agent.
Keywords: Anti urolithiasis, ethylene glycol, Azima tetracantha, calcium, phosphate,
oxalate, magnesium, uric acid, urea, creatinine
Publication date: 01/07/21
https://ijbpas.com/pdf/2021/July/MS_IJBPAS_2021_5546.pdf
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https://doi.org/10.31032/IJBPAS/2021/10.7.5546
