ABSTRACT

Galantamine isa traditional herb has also been explored for a number of pharmacological effects. Today, galantamine has been observed for its nootropic effect. There is affluence of data regarding supporting the effect of Galantamineon human neuronal cells. The present study was designed to investigate the alteration in antioxidant defense system and nootropic effects after Galantamine exposure on human neuroblastoma cells IMR-32. The treatment of Galantamine was given for 24 h and cytotoxicity study was carried out by trypan blue dye exclusion assay. Apoptosis and necrosis were observed using Propidium iodide (PI) and Hoechst double staining method. Biochemical assays like total protein, protein carbonyl, lipid peroxidation and glutathione level were analyzed along with enzymatic activity of super oxide dismutase and catalase. Result of cytotoxicity showed dose dependent increase in percent viability and significant decrease was observed in percent of apoptosis and necrosis. Moreover, exposure of Galantamine significantly decreased lipid peroxidation and protein carbonyl formation along with the enhancement in antioxidant defense mechanism. Findings of these dose reliant toxicity study of Galantamine suggest that Galantamine has higher potency as a nootropic and causes repair of human neuronal cells IMR-32 cells enhancing the cell viability and consumption ofgalantamine will help in prevention of CNS disorders and neurodegeneration. Keywords: Nootropic, IMR-32, Galantamine, Neuroprotection
Publication date: 01/07/21

https://ijbpas.com/pdf/2021/July/MS_IJBPAS_2021_5534.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.7.5534