ABSTRACT

Biosurfactants are surface-active biomolecules that show large number of applications in pharmaceutical, food, cosmetic, pesticide and oil industries, and an immense potential as an environment protective agent. In the current study, various swabs were collected from grimy surfaces, and a sludge sample was obtained from Vashi creek, Navi Mumbai. They were screened for presence of biosurfactant producers based on visual foam production in growth media and oil spreading assay. Among the 23 isolates obtained, Raoultella ornithinolytica showed maximum oil spreading potential, and surface tension reduction to 67dynes/cm. Different hydrocarbon sources like petrol, used engine oil and liquid light paraffin were used as a nutrient source for biosurfactant production. The degraded hydrocarbons were detected using standard chemical assays and identified as aldehydes, ketones and carboxylic acids. The biosurfactant also showed effective bio-degreasing and bio-destaining properties which was evaluated using the plastic surface coated with paraffin, and cotton fabrics stained with sauces, gravy, mud, lipstick, grease and methyl red respectively. Keywords: Biosurfactant, Raoultella ornithinolytica, Bio-degreasing, Bio-destaining
Publication date: 01/06/21

https://ijbpas.com/pdf/2021/June/MS_IJBPAS_2021_5508.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.6.5508