ABSTRACT

Stress affects health thereby its production in aquaculture, and fish are simultaneously exposed to multiple stresses. Here, we performed network-based gene clustering to characterize regulatory gene networks and metabolic pathways which unveil the relationships between multiple abiotic stress responsive genes.To analyze multiple responses, we performed metaanalysis and identified 1214 genes were upregulated, and 670 genes were down-regulated in zebrafish.The database for annotation, visualization, and integrated discovery was employed for functional analysis of the differentially expressed genes, PPI modules and WGCNA coexpression modules. WGCNA identified 12 modules enriched significantly in multiple stress response.These modules are enriched for genes involved in controlling the immune response, endocrine response, carbohydrate metabolism, antioxidant response, stimulus response, homeostasis,and apoptosis. These results extend our knowledge of the multiple stress responses of fish at the molecular level and indicate that fish have evolved common mechanisms to cope with multiple stresses. . Keywords: Multiple stressors; Meta-analysis; WGCNA; PPI modules; Zebrafish
Publication date: 01/06/21

https://ijbpas.com/pdf/2021/June/MS_IJBPAS_2021_5501.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.6.5501