ABSTRACT

The present research aims to select and analyse lovastatin from fungi, isolated from different soil samples. Lovastatin is competitive inhibitor of cholesterol biosynthesis by blocking 3- hydroxy, 3-methylglutaryl coenzyme A reductase (HMG-CoA reductase). Five isolates of the genus Aspergillus were isolated from different soil samples and Aspergillus terreus is an MTCC 479 strain which has been used as a standard were screened for lovastatin production in submerged fermentation for 11 days. After 11 days of fermentation, all of them showed positive results, when screened through plate assay by determining the zone of inhibition, which revealed by the fungus strain against S. cerevisiae. Lovastatin production was further confirmed by thin layer chromatography. It was found by colorimetric estimation that Aspergillus sp. (Isolate L3) to be the best producer of lovastatin upto 133.33 mg/L in submerged fermentation where as in solid state fermentation ( SSF ) using wheat bran as substrate highest lovastatin production by Aspergillus sp. (Isolate L3) was found 15.625 mg/g. High Performance liquid chromatography (HPLC) analysis found that produced lovastatin from Aspergillus sp. (Isolate L3) has the same retention time with the standard lovastatin i.e., 12.4 minutes. In total, we have isolated and screened 5 (L1 to L5) lovastatin producing cultures, among which Isolate L3 was found to be the best. Key words: Lovastatin, competitive inhibitor, submerged and solid state fermentation, HPLC analysis
Publication date: 01/06/21

https://ijbpas.com/pdf/2021/June/MS_IJBPAS_2021_5500.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.6.5500