ABSTRACT

In the present study, synthesis of silver nanoparticles (AgNPs) using leaves and stem bark of Pterocarpus indicus were compared by studied for alpha amylase, alpha glucosidase inhibition assay and also examined for its antioxidant activities by using free radical 1,1 diphenyl -2 picrycl hydrozyl DPPH scavenging method under in vitro model separately. These synthesized silver nanoparticles were characterized using UV-visible spectroscopy, Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscope (SEM), X-ray Diffraction (XRD) and zeta potential for the further confirmation. The synthesized silver nanoparticles were checked with the colour variation and it was confirmed by UV-vis spectral analysis. The morphology of the synthesized nanoparticles was analyzed using SEM. The XRD was done to find out the crystalline structure of the compound. FTIR measurements are carried out to identify the possible biomolecules responsible for capping and efficient stabilization of the silver NPs synthesized using Pterocarpus indicus. The result revealed that the leaves-AgNPs showed significant inhibitory effect on alpha amylase (77.1%) and for the stem bark-AgNPs (74.2%). Alpha glucosidase showed inhibitory effect on leaves-AgNPs (77.5%) and for stem bark-AgNPs (75.7%) were compared with standard acarbose drug. The antioxidant activities by DPPH method showed activity for leaves-AgNPs (70.15%), and for the stem bark-AgNPs (75.92%) were compared with standard ascorbic acid by measuring the percentage inhibitory effect at the concentration of 100?g/ ml respectively. Maximum inhibition was observed for reducing power assay at (79.27 %) for stem bark and for leaves (78.37 %) and compared to the standard drug ascorbic acid (84.68 %) at a concentration of 100 ?g/ml respectively. Therefore, it is suggested that the synthesized silver nanoparticles (AgNPs) using leaves and stem bark of Pterocarpus indicusis a potential source for natural antidiabetic and antioxidant compounds and could have potential use in the management of diabetes mellitus. Keywords: leaves-AgNPs and stem bark-AgNPs of Pterocarpus indicus, alpha amylase inhibitory activity, alpha glucosidase inhibitory activity, antioxidant (DPPH) activities
Publication date: 01/06/21

https://ijbpas.com/pdf/2021/June/MS_IJBPAS_2021_5492.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2021/10.6.5492