ISOLATION AND IDENTIFICATION ISOLATES OF BIFIDOBACTERIUM FROM HUMAN BREAST MILK AND THEIR USE AS PROBIOTICS Authors: Andhare P , NAYAK A, UPADHAYAY D, MARCHAWALA F, BHATTACHARYA I
ABSTRACT
Several studies have demonstrated a diversity of bacterial species in human milk, even in
aseptically collected samples. The present study evaluated potential probiotic bacteria
isolated from human milk and associated maternal variables. Milk samples were collected
from healthy women and cultured on selective and universal agar media under aerobic and
anaerobic conditions. Traditionally, human milk was thought to be a sterile fluid, but recent
findings detected the presence of biotic component in human milk, such as bacteria, including
the potentially protective genus of Bifidobacterium [1, 2]. Interestingly, these bacteria are
present in maternal milk in sufficient population levels to allow the colonization of the
newborn intestine after its ingestion, but not to induce an inflammatory response in the
mammary glands. Additionally, maternal milk contains protein for bifidobacteria, the socalled
Bifidus factors which are constituted of a spread of oligosaccharides in quite high
concentrations (14 to 20 g/L). In this way, human milk can be considered as a natural
symbiotic containing both probiotics (Bifidobacterium) and prebiotics (human milk
oligosaccharides: HMO) in its constitution [3]. For this reason, breastfeeding is nutritional,
immunologically and microbiologically essential for newborn.
Keywords: Bifidobacterium dentium, Streptococcus thermophilus, Lactobacillus bulgaricus, Bifidobacterium bifidus Publication date: 01/04/21 https://ijbpas.com/pdf/2021/April/MS_IJBPAS_2021_APRIL_SPCL_1001.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2021/10.4.1001