ISOLATION AND IDENTIFICATION ISOLATES OF BIFIDOBACTERIUM FROM HUMAN BREAST MILK AND THEIR USE AS PROBIOTICS
Authors: Andhare P , NAYAK A, UPADHAYAY D, MARCHAWALA F, BHATTACHARYA I

ABSTRACT
Several studies have demonstrated a diversity of bacterial species in human milk, even in aseptically collected samples. The present study evaluated potential probiotic bacteria isolated from human milk and associated maternal variables. Milk samples were collected from healthy women and cultured on selective and universal agar media under aerobic and anaerobic conditions. Traditionally, human milk was thought to be a sterile fluid, but recent findings detected the presence of biotic component in human milk, such as bacteria, including the potentially protective genus of Bifidobacterium [1, 2]. Interestingly, these bacteria are present in maternal milk in sufficient population levels to allow the colonization of the newborn intestine after its ingestion, but not to induce an inflammatory response in the mammary glands. Additionally, maternal milk contains protein for bifidobacteria, the socalled Bifidus factors which are constituted of a spread of oligosaccharides in quite high concentrations (14 to 20 g/L). In this way, human milk can be considered as a natural symbiotic containing both probiotics (Bifidobacterium) and prebiotics (human milk oligosaccharides: HMO) in its constitution [3]. For this reason, breastfeeding is nutritional, immunologically and microbiologically essential for newborn. Keywords: Bifidobacterium dentium, Streptococcus thermophilus, Lactobacillus bulgaricus, Bifidobacterium bifidus
Publication date: 01/04/21
    https://ijbpas.com/pdf/2021/April/MS_IJBPAS_2021_APRIL_SPCL_1001.pdf
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https://doi.org/10.31032/IJBPAS/2021/10.4.1001