OPTIMIZING THE MANAGEMENT OF LEAD BIOACCUMULATION CAPACITY OF METAL-RESISTANT PSEUDOMONAS STUTZERI (IC29 MK965194) ISOLATED FROM THE E-WASTE CONTAMINATED SOIL SAMPLES Authors: Pradeepa R* And Kavitha KK
ABSTRACT
E-waste is major environmental pollutants when present in soil and show potential toxic effects
on growth and development in plants and animals. E-waste contains a number of toxic materials like
lead, mercury, cadmium, hexavalent chromium and brominated flam. Most of the metal tolerant microbes
as used for the remediation, this point of view, and the present investigations were carried out to isolate
metal tolerant bacterial species from the E-waste contaminated sites on Trichy area. Totally 44 colonies
were observed from 3 soil samples, among them 6 bacterial genera and 9 species were obtained. Among
the 44 isolates, single isolate was tolerating up to 14mM concentration of lead. This isolate was confirmed
as Pseudomonas stutzeri by using 16srRNA sequence. The bioaccumulation of started with media
optimization and results showed maximum accumulation of heavy metal (lead) at 37°C temperature, pH
7.0, lactose and yeast extract as carbon and nitrogen source respectively. The results demonstrated that
under such ideal condition bioaccumulation of Pb by Pseudomonas stutzeri was found to be at a
maximum of 1144?g accounting to 39.4%.
Keywords: - E-waste, Metal, Lead, Bioaccumulation, Pseudomonas stutzeri Publication date: 01/05/21 https://ijbpas.com/pdf/2021/May/MS_IJBPAS_2021_5458.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2021/10.5.5458