ABSTRACT

Bio-nanotechnology is the most emerging area of research these days due to its immense potential and applications and is making an impact in all spheres of human life. Hence the present investigation was carried out to synthesize Zinc oxide nanoparticle using bacteria, Escherichia coli, to study the characterization and antibacterial activity of synthesized Zinc oxide nanoparticle. Biosynthesis of Zinc oxide nanoparticle using bacteria, E.coli and Zinc acetate dehydrate solution with ? 99.0% purity was carried out. Characterization of nanoparticle was carried out by FTIR analysis, (SEM) and (TEM) and antibacterial activity of biosynthesized Zinc oxide nanoparticle was carried out by well diffusion method. The results of characterization of Zinc oxide nanoparticle by FTIR analysis of the biosynthesized nanoparticle revealed the presence of biomolecules in the nanoparticle that are responsible for the reduction and stabilization process for the biosynthesis of nanoparticle. The results of the SEM studies showed rectangular and cuboidal shaped nano stuctures and Zinc oxide nanoparticle without any aggregation were observed in the TEM image. The results of the antibacterial activity of biosynthesized Zinc oxide nanoparticle using E.coli, against the test organisms (Bacillus sp., Staphylococcus epidermidis and Staphylococcus saprophyticus) carried out by well diffusion method revealed that Zinc oxide nanoparticle had significant inhibitory effect against all the test bacterial species but slightly higher rate of inhibition was recorded in Staphylococcus saprophyticus (29 mm) followed by Bacillus sp. (27mm) and Staphylococcus epidermidis (23mm). Thus from the results of the present study, it can be concluded that biosynthesis of Zinc oxide nanoparticle using supernatants of E.coli has several advantages such as simple, cost effective, less time consuming, safe and ecofriendly compared to physical and chemical methods of nanoparticle synthesis and biosynthesized Zinc oxide nanoparticle has a potent antibacterial activity as evidenced in the present study. Keywords: Biosynthesis, E.coli, Zinc oxide nanoparticle, characterization, antibacterial activity, well diffusion method
Publication date: 01/05/21

https://ijbpas.com/pdf/2021/May/MS_IJBPAS_2021_5449.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.5.5449