ABSTRACT

Cotton (Gossypium hirsutum) is one of the largest source of natural fiber that’s why its production is required in enormous quantity, but different environmental stresses cause the lost in the yield. Genetic engineering has enabled us with various techniques to overcome the losses and fulfilling the human needs through modifying the genetics of plant cells. In this study a variety of cotton plant was modified using Agrobacterium tumefaciens with BAR gene. BAR gene was inserted to resist the effect of herbicide bialaphos, which affects the primary growth of plants. The whole study was conducted at Four Brothers genetics. The Bar gene cassette, seeds and all the necessary material was obtained from Four Brother genetics. The construct was already made and it was just transformed to A. tumefaciens. The seed were delinted, soaked and BAR (Bialaphos resistant gene) was transformed to germinated embryos. After the step by step growth of embryos into healthy plants, molecular analysis for transformation of BAR gene was confirmed through leaf sample of stable transformed plants. Transformation was determined by PCR and gel electrophoresis. The entire study confirms that transgenic cotton variety of eagle-2 shows better results of transformation for BAR gene than other varieties. Keywords: Cotton, A. tumefaciens, herbicide resistance, bialaphos, BAR gene, PCR
Publication date: 01/04/21

https://ijbpas.com/pdf/2021/April/MS_IJBPAS_2021_5465B.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.4.5465B