ABSTRACT

Gymnema sylvestre R.Br. belongs to asclepiadaceae family, used for the treatment of diabetes. Gymnemic acid, a group of related triterpenoid compounds present in leaf are the active molecules responsible for the biological properties of G. sylvestre. The present study was carried out to investigate the influence of different doses of elicitors such asketoconazole, chitosan, methyl jasmonate (MeJA) and salicylic acid (SA) on cell growth and gymnemic acid production profiles in cell suspension cultures of G. sylvestre. Among the elicitors tested, SA at 100?M significantly increased the cell growth (172±5.7%) versus control (144±3%). All the elicitors even at lower concentrations improved gymnemic acid content than their higher dose counter parts. Based on HPLC analysis, higher elicitation efficiency in the form of gymnemic acid production was observed with MeJA at 50?M and is 8 folds higher (84.6±3.2mg/g DW) compared with control. Results suggest that, elicitor induced cell stress is imperative for the enhanced gymnemic acid production. Overall, MeJA at 50?Mcould be used as suitable elicitor dose for effective growth and gymnemic acid production. The results showed that, cell suspension cultures combined with optimised elicitor doses has potential to improve the gymnemic acid production at large scale in a cost-effective manner. Keywords: Gymnemic acid, Elicitors, Methyl jasmonate, HPLC, Cell growth
Publication date: 01/03/21

https://ijbpas.com/pdf/2021/March/MS_IJBPAS_2021_5719.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.3.5719