For a wide range of diseases, age itself is a single and important risk factor. During the process
of aging, there is gradual and constant accumulation of oxidized proteins take place inside the
cells because of reduction in tolerance to physiological stress. Aging is also related to changes in
hormonal levels of HPG axis. In aged males, reduction of testicular functions along with a
decline in reproductive functions arises year by year. Oxidative stress has a main role in process
of aging. The study is carried out on a total of healthy male subjects, ages between 21 years to
100 years. This is a comparative study between younger group and older group. All the samples
were collected from Bahawalpur, Pakistan. Physical measurements and blood collection was
done according to WHO criteria. To study the changes and effects, serum levels of LH, FSH,
SHBG, testosterone and 8OHdG were used.The mean values of Hemoglobin (14.62±1.44 g/L),
lymphocytes (32.97±9.16 %), eosinophils (5.13 ±4.28 %) and HCT (46.19 ±4.40 %) were
significantly increased in age group (41-60) as compared to other age groups. On the other hand, the mean values TLC (9.43± 7.35 %), monocytes (11.45 ±1.90 %) and MCV (91.00 ±3.67 %)
were higher in older age groups (81-100) as compared to other age groups. Whereas, the mean
values of RBC, MCHC and platelets were significantly increased (5.83±0.66 s/L, 32.00 ±1.65%
and 295.55±79.70) in the age group (21-40) respectively, as compared to other age groups.The
mean value of SHBG was significantly increased (53.25±27.24 nmol/l) in older age group (81-
100) as compared to other groups with P-value of (0.02). Apart from that, the mean values of LH
(9.79±8.27 mIU/ml) and FSH (18.23±29.43 mIU/ml) and 8-OHdG (1.15±0.058 ng/ml) were also
higher in older age group (81-100) as compared to other age groups. On the other hand, the mean
value of testosterone was significantly increased (530.60±168.74 mIU/ml) in age group (21-40)
as compared to other groups. In the present study, 8-OHdG has been used not only as a
biomarker for the estimation of endogenous oxidative DNA damage, but also a major risk factor
to cause ageing in males. From the gonadal axis profile, the sex hormone binding globulin
(SHBG) has a significant role in the ageing process. Alteration of SHBG has strongly linked with
obesity, prostate hypertrophy, sexual activity, cardiovascular disease and HDL-cholesterol. In
conclusion, SHBG is a potential physiological biomarker to control aging.
Keywords: Ageing, FHS, LH, SHBG, Total Testosterone, 8-OHdG
Publication date: 01/03/21
https://ijbpas.com/pdf/2021/March/MS_IJBPAS_2021_5423.pdf
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https://doi.org/10.31032/IJBPAS/2021/10.3.5423
