ABSTRACT

Most of the Indian distilleries utilize molasses as raw material for the production of alcohol. Alcohol production from molasses generates large volumes of high strength unwanted residual liquid called spent wash. The distillery spent wash pose a major threat to soil and aquatic ecosystem due to the presence of water soluble recalcitrant colouring compound called melanoidin. Therefore, adequate treatment is necessary before the discharge of distillery spent wash into the environment. The present study was undertaken for investigation of decolourization potential of Lactobacillus plantarum ABS0S11 isolated from soil. Different environmental and nutritional parameters were optimized for decolourization and degradation of distillery spent wash. Lactobacillus plantarum ABS0S11 showed maximum decolourization (41.3 %) in presence of glucose (1%, w/v) and peptone (0.3%, w/v) at pH 6.0 and temperature 350C under static condition. The ability of the bacterial strain to decolorize and degrade the spent wash was confirmed by High Performance Liquid Chromatography analysis. Further, phytotoxicity assay of distillery spent wash with Phaseolus mungo L. showed a reduction of toxicity in bacterial treated spent wash sample. Keywords: Lactobacillus plantarum, decolourization, spent wash, static condition
Publication date: 01/03/21

https://ijbpas.com/pdf/2021/March/MS_IJBPAS_2021_5418.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.3.5418