ABSTRACT

Kodagu is a tiny piece of land located in the heart of Western ghats. Epiphitic orchid, Cleisostoma tenuifolium from this were used for our study. Similar to all orchids, this is also a flowering plant with yellow charming flowers. We have identified five endophytic bacterial strains from the leaves of this plant by molecular identification namely Bacillus subtilis, Psychrobacillus psychrodurans, Bacillus cereus, Bacillus safensis, Bacillus pumilus. The study revealed that all of them are bacillus but, are different from each other. Common protocols were used for isolation and 16S rDNA sequencing following PCR were used for genotypic characterization of bacteria. Obtained sequences were compared for with known bacterial culture from NCBI database and submitted to GenBank. Homology of the bacteria were studied using Phylogenetic tree analysis. Future studies were needed to identify more endophytes from different parts of this vulnerable plant and thereby a small effort for conservation of biodiversity. Keywords: Endophytic bacteria, 16S rDNA, PCR, GenBank, orchid, biodiversity
Publication date: 01/03/21

https://ijbpas.com/pdf/2021/March/MS_IJBPAS_2021_5401.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.3.5401