ABSTRACT

The aim of present study was to identify and Characterize Reserpine in Rauwolfia serpentina (Indian snakeroot) and gallic acid in Phyllanthus emblica (Amla) in polyherbal formulation. Dried roots of rauwolfia serpentine and amla fruits were taken and were extracted for their constituents by Soxhelt apparatus [1]. The extract was dried using rotary film evaporator. Extract powder was taken and phytochemical tests were performed and results showed that alkaloids and vitamins were present along with many other constituents. These powders of plants along with polyherbal formulation were taken and solubility studies were carried out. Identification and characterization of constituents was done using sophisticated analytical technique HPTLC. Stationary phase used was Pre-coated aluminium TLC plates with silica gel 60F254 of 10 X 10 cm size and mobile phase was Toluene: Ethyl acetate: Formic acid: Methanol (3:3:0.8:0.2) with 8 ?L/sec spraying rate of sample and detection was done at 254nm and 365nm respectively. Rf values 0.963 and 0.326 respectively. Hence we conclude that Reserpine and gallic acid are the major active constituents present in these plants. Keywords: HPTLC, Rauwolfia serpentine, Phyllanthus emblica
Publication date: 01/03/21

https://ijbpas.com/pdf/2021/March/MS_IJBPAS_2021_5394.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.3.5394