EVALUATION OF ANTIMICROBIAL ACTIVITY AND MIC OF TRADITIONALLY USED MEDICINAL PLANT AZADIRACHTA INDICA AGAINST OPPORTUNISTIC HUMAN PATHOGENIC MICROORGANISMS Authors: Giri RP , GANGAWANE AK, AND GIRI SG
ABSTRACT
Antimicrobial activity in leaf extract of neem (Azadirachta indica) against human pathogenic
microorganism: Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli,
Enterococcus faecalis, Candida albicans. Antimicrobial activities of aqueous, methanol and
ethyl acetate extracts of neem leaves were used. Varying concentration of each extracts 500
mg/ml, 250 mg/ml, 125 mg/ml, 62.5 mg/ml, 31.25 mg/ml, 15.625 mg/ml prepared in DMSO and
tested using well diffusion method and also checked for MIC. When compared with ampicillin-
20mg/ml, the Ethyl Acetate extract shows highest zone of inhibition followed by methanol
extract and aqueous extract on Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia
coli, Enterococcus faecalis, Candida albicans in an ascending order.
Keywords: Azaridichta indica, Staphylococcus aureus, Pseudomonas aeruginosa,
Escherichia coli, Enterococcus faecalis, Candida albicans, microbiota Publication date: 01/02/21 https://ijbpas.com/pdf/2021/February/MS_IJBPAS_2021_5339.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2021/10.2.5339