ABSTRACT

This study was undertaken to validate the efficacy by evaluating the hepatoprotective activity of ethanolic root extract of Dracaena terniflora Roxb.(DTR) on Hep G2 cell line. Four doses of DTR root extract (6.25, 12.5, 25, 50 and 100 ?g/ml) were tested on ethanol induced HepG2 cell toxicity model. The cytotoxicity study was conducted on the DTR root extract, using MTT assay to determine the CTC 50 value which was 188.81%. The ethanolic root extract was also evaluated for their hepatoprotective activity on human liver hepatocellular carcinoma (Hep G2) cell line against ethanol as a liver damage inducing agent by quantifying the release of lactate dehydrogenase (LDH) in the medium. Of all the DTR treated groups, the Hep G2 cell lines treated with 6.25 ?g/ml concentration showed maximum cell viability (91.79%) and minimum LDH leakage (0.0401U/ml). The DTR root extract exhibited significant (P < .001) cytoprotective activity with a maximum protection of 91.79 % at dose 6.25?g/ml, and the activity was comparable with that of the standard, Silymarin. The results of the present study indicate that the Dracaena terniflora Roxb. demonstrated a significant hepatoprotective activity and could be used as an active herbal alternative for the treatment of liver ailments. Keywords: Dracaena terniflora Roxb. ethanol, HepG2, MTT, hepatocytes, Hepatoprotective activity, Silymarin
Publication date: 1/1/21

https://ijbpas.com/pdf/2021/January/MS_IJBPAS_2021_5676.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.1.5676