ABSTRACT

Carboplatin is available in injection dosage form; therefore a reliable and economical method is required for its analysis for quality control. The objective of the present study is to develop and validate UV-spectrophotometric method for analysis of Carboplatin and its comparison with already existing method of high performance liquid chromatographic method. The purpose of this comparison is to evaluate the added significance of using HPLC method in the presence of validated UV method. The validated UV-spectrophotometric method demonstrated an excellent linearity (r2 = 0.9995) in a range of concentration from 10?g/mL to 50?g/mL, precision (%RSD = 0.388) and a mean percentage recovery of 100.14% indicating an accurate method. The assay results of Carboplatin injection obtained from HPLC and UV-spectroscopic methods are quite comparable. Since no added benefit of HPLC method is found in the presence of validated economical UV-spectrophotometric method, therefore for the quality control purpose, the UV method may be used for analysis of Carboplatin API as well as in injectable dosage form as an alternative to HPLC. Key words: Carboplatin, UV-spectrophotometry, HPLC, ICH, Sodium hydroxide
Publication date: 1/1/21

https://ijbpas.com/pdf/2021/January/MS_IJBPAS_2021_5334.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.1.5334