ABSTRACT

The present study aims to screen the in vitro cytotoxicity, antioxidant potential and hepatoprotective activity of hexane (AVH), ethyl acetate (AVE) and methanol (AVM) extracts of Alysicarpus vaginalis var. nummularifolius, which is a traditionally used plant for jaundice. The cytotoxicity screening of different extracts was determined by MTT cell viability assay using L929 normal fibroblast cell lines. Antioxidant potential of plant extracts was studied by ABTS and hydroxyl radical scavenging activity. Total antioxidant capacity was evaluated by phosphomolybdenum method. In vitro hepatoprotective activity of the extracts was evaluated against CCl4 induced toxicity in HepG2cell lines. Invitro cytotoxicity study report the IC50 values of hexane, ethyl acetate and methanol extracts of A.vaginalis as 55.26?g/ml,41.51?g/ml and 112.6?g/ml respectively. Significant dose dependent increase in percentage viability of HepG2 cells at the dose 6.25, 12.5,25,50 and 100?g/ml concentration of different extracts compared to CCl4 (0.1%) exposed cells. AVM showed high hepatoprotective activity than AVH. AVE showed dose dependent action, lower concentrations give hepatoprotective activity while at higher concentration an inhibitory effect is noticed. Thus the present study shows that methanol extract is non-toxic in nature while hexane and ethyl acetate extract of A.vaginalis are moderately toxic. High antioxidant activity is exhibited by ethyl acetate and methanol extract than the hexane extract. A good hepatoprotective effect is exhibited by the methanol extract than the other two extracts. Keywords: Alysicarpus vaginalis var. nummularifolius, in vitro cytotoxicity, hepatoprotective activity, antioxidant
Publication date: 1/1/21

https://ijbpas.com/pdf/2021/January/MS_IJBPAS_2021_5298.pdf

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https://doi.org/10.31032/IJBPAS/2021/10.1.5298