OXIDANT AND ANTIOXIDANT STATUS DURING DIFFERENT STAGES OF LACTATION IN NILLI-RAVI BUFFALOES AND SAHIWAL COWS Authors: Naeem M , NISA ZU AND RAHMAN ZU
ABSTRACT
Lactation is an important period in dairy animals during which many physiological changes takes
place and produced a measureable changes in diagnostic parameters in blood. Stages of lactation
also affect the oxidative status of animals. Stress expressed by dairy cattle during lactation is a
challenged situation. The present study was aimed to assess the status of oxidants and
antioxidants status in Nili Ravi buffaloes and Sahiwal cows during different stages of lactation.
Lactating buffaloes (n=30) and cows (n=30) were randomly selected from two different farms
and categorized into three stages, stage I (n=10), stage II (n=10) and stage III (n=10). From each
stage blood sample were drawn. Melanodialdehyde (MDA) was determined by measuring serum
lipid peroxidation (LPO), while serum enzymes were measured by applying standard
methodologies and using spectrophotometer (Biosystem BTS-330). Non significantly lower
concentration of TAS was found in stages I and stage III of lactation. TOS, SOD and catalase
were significantly higher at lactation stage II. MDA and Ceruloplasmin were significantly higher
at lactation stage II in buffaloes and lactation stage III in cows. These parameters assist to
describe the changes in physiology of animals which is associated with oxidative stress during
lactation.
Keywords: Oxidative stress, antioxidants, lactation, buffaloes and cows Publication date: 01/12/2020 https://ijbpas.com/pdf/2020/December/MS_IJBPAS_2020_5316.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2020/9.12.5316