EVALUATION OF OXIDATIVE STRESS AND ANTIOXIDANT MARKERS DURING VARIOUS STAGES OF LACTATION IN SMALL RUMINANTS Authors: Naeem M , KANWAL S, REHMAN ZU
ABSTRACT
The present study was conducted to evaluate the effect of oxidative stress through various
antioxidative/oxidative systems during different lactation stages in sheep and goats. A total of 60
lactating ewes and does between 1.5 to 5 years age group comprised the experimental animals
taken from Bahadar Nagar Farm, Okara. Thirty (30) female Lohi sheep and likewise 30 female
Beetal goats were grouped into three different milking stages [lac I (n=10), lac II (n=10) and lac
III (n=10)]. Blood samples were collected from the animals during three different lactation
periods. Serum has been preserved at -20 0C and used for determination of Malondialdehyde
(MDA), Superoxide Dismutase (SOD), Catalase (CAT), Ceruloplasmin (Cp), Aspartate
Aminotransferase (AST), Alanine Aminotransferase (ALT) and Gamma-glutamyl Transferase
(GGT). Statistical analysis was done by two way ANOVA and Duncan Multiple Range Test
(DMRT). MDA, SOD, CAT, Cp and GGT were significantly different (P?0.05) through various
stages of lactation in sheep as well as in goats. Whereas, AST and ALT were not differ
significantly during different stages of lactation in both the groups. In Conclusion, lactation may cause an increase in reactive oxygen species and antioxidant substances predicting that is very
stressful for the animals.
Keywords: Oxidants and antioxidants, Lactation, Liver Enzymes, Sheep, Goat Publication date: 01/12/2020 https://ijbpas.com/pdf/2020/December/MS_IJBPAS_2020_5314.pdfDownload PDFhttps://doi.org/10.31032/IJBPAS/2020/9.12.5314