ABSTRACT

Periodontitis is a gum infection with symptoms like gum inflammation, tooth loss etc. caused due to bacteria residing in biofilms at or below the gingival margin. Control remedies like tablets and surgical methods are used but have their own side effects like liver toxicities and postoperative complications. Widespread number of local drug delivery systems have been introduced one of them being nanoparticles having significant surface area and sustained release properties at affected site. In our experiment we have formulated ciprofloxacin (CF) nanoparticles using PLGA as polymer further coated with chitosan (CH) by single emulsification method and carried out their evaluation and optimization. Evaluation parameters like size & morphology, in-vitro drug release, mucoadhesion studies, DSC, irritation testing and stability studies were carried out to achieve best of the conditions for nanoparticle formulation. Since, ciprofloxacin is a second generation fluoroquinolone antibiotic with 3-4h shelf life, 1?g/ml or less minimum inhibitory concentration (MIC),effective against both gram positive and gram negative bacteria act as a potent drug for nanoparticle formulation for periodontitis. Single Emulsification method resulted in smaller sized ciprofloxacin nanoparticles (NPs). Moreover, results of in-vitro drug release showed that chitosan coated ciprofloxacin PLGA NPs (CH-CF-PLGA-NPs) have sustained drug release profile. CH coated nanoparticles were found to be non irritant and show good mucoadhesion. Hence prepared CH-CF-PLGA-NPs found to be suitable for dental carries like periodontitis. Keywords: Chitosan, Ciprofloxacin, Mucoadhesion, Periodontitis, PLGA Nanopartciles, Single Emulsification
Publication date: 01/12/2020

https://ijbpas.com/pdf/2020/December/MS_IJBPAS_2020_5286.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.12.5286