ABSTRACT

Increasing contamination of soil with hydrocarbon due to the activities of the chemical and oil refinery industry has prompted severe environmental pollution. The biodegradation of hydrocarbon compounds by the microorganisms has shown promising results to overcome this problem. In the present study, we found stabilized bacterial consortium from the oil contaminated soil samples in Namakkal, Tamilnadu, India. Presently, 6 isolates were showed positive result in all primary screenings methods. Among them, single isolate of Citrobacter freundii was potential isolate, which was confirmed with 16sr RNA sequencing. The media has been optimized with potential isolate and increasing the biosurfactant level. Furthermore, the biosurfactant has been identified as a way to reduce hydrocarbon contamination from soil. This study further identified the direct proportional relationship between biosurfactant production and operational variables, showing that biosurfactant are a cheap and viable production pathway map. Keywords: Biosurfactant, Bioremediation, Citrobacter freundii, Hydrocarbon, CTAB, gravimetric method
Publication date: 01/12/2020

https://ijbpas.com/pdf/2020/December/MS_IJBPAS_2020_5272.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.12.5272