ABSTRACT

Amphibian skin glands provide variety of compounds as a source of potential new chemical entities. Indian Toad Paratoid Glandular Secretions (TPGS) show anti-diabetic and cardio tonic activities and limited research is done on other activities. The present study focused to explore the antioxidant, anti-inflammatory effects. Male Wistar rats (n=6) used for acute toxicity study according to OECD 423 guidelines. In-vitro and in-vivo antioxidant study was performed by DPPH and CCl4 induced oxidative stress model respectively. Further, anti-inflammatory with paw edema and absorption kinetics of naproxen was done and LD50 found to be 500 mg/kg. TPGS shown potential antioxidant effect with IC 50 value of 33.53±1.26?g/ml. Significant decrease in serum enzymes and MDA levels and increase in GSH levels observed in TPGS and ascorbic acid pretreated rats when compared with CCL4 induced rats and was evidenced with histopathological study. Percentage reduction of rat paw edema was 47.6±1.3 with TPGS alone and 49.6±0.9 was with TPGS + NPX. In the pharmacokinetics study between naproxen and TPGS shows significant decrease in Cmax, AUC, and an increase in Cl and Vd indicating the induction of NPX metabolism. Based on the results TPGS has anti oxidant, anti-inflammatory and enzyme induction effects. So TPGS can be a good source for the potential new chemical compounds for different biological activities. Further studies are required to prove the activities. Keywords: Acute toxicity study, Ascorbic acid, Bufo melanostictus, Carrageenan, Inflammation, Naproxen
Publication date: 01/12/2020

https://ijbpas.com/pdf/2020/December/MS_IJBPAS_2020_5267.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.12.5267