ABSTRACT

The aim of this study was to carry out phenotypic detection of ESBL and AmpC producing Pseudomonas aeruginosa by carrying out the following in vitro antimicrobial assaysscreening of isolates for ESBL production, Confirmation of ESBL production by Double Disc Synergy Test (DDST), Modified Double Disc Synergy Test (MDDST), Screening of isolates for AmpC production, Disc antagonism test and ESBL and AmpC E – test .The results of the study showed that (50%) isolates were ESBL positive by (DDST) and (52.4%) by (MDDST). (61.9%) isolates were positive for AmpC production by screening method. (61.9%) and (57.1%) isolates were positive for AmpC by disc antagonism method and (37.3%) were found positive for both ESBL and AmpC production by ESBL and AmpC Etest. Hence the present investigation was carried out to find the frequency of ESBL and AmpC production in clinical samples of Pseudomonas aeruginosa isolates. Keywords: Double Disc Synergy Test (DDST), Modified Double Disc Synergy Test (MDDST), Disc antagonism test, ESBL, AmpC, E – test, Multidrug resistance, Pseudomonas aeruginosa
Publication date: 01/11/2020

https://ijbpas.com/pdf/2020/November/MS_IJBPAS_2020_5277.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.11.5277