ABSTRACT

Objective: The aim of the present investigation was to extract various phytoconstituents from Ocimum tenuiflorum leaf and Zingiber officinale stem using ethanol, chloroform, and n-butanol as solvent and characterized for biomedical applications. Material and methods: Obtained extracts were used for phytochemical screening and characterized in terms of ash value determination, TLC and FTIR spectral analysis. Various concentrations of extracts viz. 1, 2, 3, 4 and 5 ?g/ml were prepared and evaluated for free radical scavenging potential against DPPH. Ascorbic acid was used as standard to compare free radical scavenging activity. Antimicrobial efficacy of extracts was also determined against Escherichia coli and Bacillus subtilis. Result and discussion: Results showed that chloroform extract of Zingiber officinale have best antioxidant activity among all the extracts. Ethanolic extract of ginger showed significantly higher efficacy against B. Subtilis while ethanolic extract of Ocimum tenuiflorum has higher antimicrobial efficacy against E. coli. Extracts were also good antioxidant activity. Conclusion: It can be concluded from the findings of the result that Ocimum tenuiflorum and Zingiber officinale have significant antioxidant and antimicrobial effect and can be used in pharmaceutical, food and cosmceuticals industry. Keywords: Ocimum tenuiflorum leaf; Zingiber officinale stem; antioxidants; free radical, herbal extract
Publication date: 01/11/2020

https://ijbpas.com/pdf/2020/November/MS_IJBPAS_2020_5230.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.11.5230