ABSTRACT

Progesterone and Estrogen are being used to synthesize Combined oral contraceptives (COCs) and serve as most convenient, safe, effective method of contraception. Due to their side effects in most women, herbal medicines have been proposed as alternatives to these contraceptive methods. The present study was aimed to evaluate the antifertility effects of Genistein (isolated from Glycine max), via in-silico and in vivo experimentation on female Rattusnorvergicus (Wistar albino rats). Genistein was docked with Estrogen Receptor of rats (ER ? and ER ?). For, antagonist actions we compared ERA-4-[(2-{4-[(1E)-1-(1H-indazol-5- yl)-2-phenylbut-1-en-1-yl]phenoxy}ethyl)amino]-N,N-dimethylbutanamide complex with ERA-genistein complex and ERB-(R,R)-5,11-cis-diethyl-5,6,11,12-tetrahydro-chrysene-2,8- diol complex (PDB ID: 1l2J) with ERB-genistein complex. To further investigate the in vivo anti-fertility effects of Genistein and its correlation with serumestrogen, adult Rattus norvergicus (Wistar albino rats) was administered with 10, 20 and30mg Genistein/kg body weight dissolved in DMSO: PBS (1:4) vehicle for 15 days. Estrogen hormone level in the serum was estimated through ELISA. In-silico observation has shown that Genistein may act as antagonist for Estrogen Receptors. Serum estrogen level was found to be reduced, which can lead to disrupt ovulation. This shows that the Genistein might be working as antiovulatory agent by acting as a phytoestrogen. Keyword: Genistein, estrogen, estrogen receptors, docking study, anti-ovulation, herbal contraceptive
Publication date: 01/09/2020

https://ijbpas.com/pdf/2020/September/MS_IJBPAS_2020_52071.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.9.5207