ABSTRACT

The biogenic synthesis of gold nanoparticle is reliable, nontoxic, economical and ecofriendly in comparison to the conventional synthesis of gold nanoparticles. The synthesis of AuNPs by E. coli MTCC40 supernatant was confirmed by absorption spectrums and electron microscopy. The synthesized NPs showed red color and absorption peak at 549 nm. On the basis of transmission electron microscopy analysis, size of gold nanoparticles was estimated 5-20 nm. In Fourier Transform Infra red spectroscopy (FTIR), observed absorption peaks corresponding to amine, amide and carbonyl group were believed to be present on the amino acid residue of protein and may be responsible for reduction and formation of AuNPs. The extracellular AuNPs were further utilized for surface modification by Self assembled monolayer of L cysteine in order to immobilize them on the gold electrode. Cysteine Modified gold nanoparticles and gold electrode were characterized by cyclic voltammeter study. Electrochemical impedance spectroscopy analysis confirmed the increase impedance with gold nanoparticles for the detection of E. coli. Keywords: Gold nanoparticles, Characterization, TEM, Biogenic
Publication date: 01/10/2020

https://ijbpas.com/pdf/2020/October/MS_IJBPAS_2020_5222.pdf

Download PDF
https://doi.org/10.31032/IJBPAS/2020/9.10.5222