Aging is a complex process of gradual deterioration of physiological functions including fertility.
Different explanations have been given, most accepted one is the oxidative stress theory of aging
and infertility. Oxidative stress is an imbalance between ROS and anti-oxidant defense system of
the humans. During the aging process, there is constant production of oxidative proteins which
causes the partial unfolding of proteins leading to aggregates. This protein aggregation leads to a
defective cellular proteolytic system which results further production of oxidative products
causing damage to macromolecules and apoptotic cell death.Fifty healthy male subjects between
the age 20-85 were included in the study. We examined the natural alterations in hypothalamic
pituitary gonadal axis in aging male of south East Asia. Normal age-related changes in serum
levels of testosterone, LH, FSH and SHBG were noted. HSP 70 relation with these observations
was established to check the age-related changes effected on general health status. A
questionnaire was used to exclude the systemic diseases, smoking and risk factors. BMI was
calculated. RBS and CBC were performed on the same day after centrifugation of sample within
two hours. All assays were performed under the standard procedures by manufacturers in the lab
by skilled staff. The hormonal assay kit was used for testosterone, LH, FSH and SHBG. The
results received and entered in excel sheets. The quantitative sandwich ELISA kit was used to
determine the HSP 70 levels in undiluted human serum samples.Maximum range of Hb was
observed in the male individual (15.48±11.02) with the age group (21-40) as compared to other groups. The mean values of MCV, MCH and MCHC were significantly high (5.45±0.31, 82.56
±5.07 and 29.20±0.00) in Group (81-100), Group (61-80) and Group (1-20)respectively, as
compared to other age groups. Nevertheless, the mean values of WBC, neutrophils, lymphocytes,
eosinophils and platelets were significantly higher (33.45± 1.77 s/L, 7.47±2.40 %, 63.50±7.72%,
1.50± 0.57 %, and 1.50± 0.57%) in older age group (81-100) correspondingly as compared to
other age groups. Contrary to that, the mean values of monocytes and RBC were significantly
increased (38.00± 0.00 % and 280.2 ±125.9s/L) in Group (1-20) and Group (41-60) respectively.
The mean value of HSP-70 was statistically significantly increased (415.26±11.08 ng/ml) in
older age group as compared to other groups with P-value 0.01. Meanwhile, the mean values of
LH and testosterone were significantly high (7.16±0.00 mIU/ml and 551.01±0.00 mIU/ml) in age
group (I-20) respectively. On the other hand, the mean values of SHBG (38.87±17.57 nmol/l )
and FSH (6.70±6.27 mIU/ml ) were increased in age Group (21-40) and Group (61-80)
respectively, with the contrary to the other age groups.In the present study, it has been concluded
that heat shock protein-70 (HSP-70) has a significant role to upregulate the production of free
radicals and oxidative stress during ageing. HSP-70 has been used not only as a biomarker for
the estimation of endogenous oxidative DNA damage, but also a major risk factor to cause
ageing in males.
Keywords: Ageing, LH, FSH, SHBG, HSP-70
Publication date: 01/10/2020
https://ijbpas.com/pdf/2020/October/MS_IJBPAS_2020_4880.pdf
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https://doi.org/10.31032/IJBPAS/2020/9.10.4880
