Objective:
The present study was designed to evaluate the in vitro antimicrobial activity of
(1R,3aR,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR)-3a,5a,5b,8,8,11a-hexamethyl-1-prop-1-en-2-
yl1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysen-9-ol (Lupeol) isolated
from methanolic leaf extract of Andrographis echioides.
Methods:
The anti microbial activity was determined by using the disc diffusion method against selected
pathogens. The isolation was done using chromatography techniques. Isolated compound was subjected
to spectral analysis. Structural elucidation was carried out by Interpretation of spectral data. The pure
isolated compound from methanolic leaf extract of Andrographis echioides was confirmed to be
(1R,3aR,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR)-3a,5a,5b,8,8,11a-hexamethyl-1-prop-1-en-2-yl-
1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysen-9-ol.
Results:
The obtained results indicated that the isolated compound
(1R,3aR,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR)-3a,5a,5b,8,8,11a-hexamethyl-1-prop-1-en-2-yl-
1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysen-9-ol was more efficient
against selected pathogens at higher concentration. The antimicrobial potential of methanolic leaf extract
of isolated compound from Andrographis echioides determined on the basis of mean diameter of zone of
inhibition around the disc in millimeters. The isolated compound
(1R,3aR,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR)-3a,5a,5b,8,8,11a-hexamethyl-1-prop-1-en-2-yl-
1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysen-9-ol exhibited
antibacterial activity against Escherichia coli, Staphylococcus aureus and Enterococcus aerogenes,
Pseudomonas aeruginosa ,Proteus vulgaris and anti-fungal activity against Candida albicans, Candida
tropicalis, Aspergillus flavus, Aspergillus niger and Candida vulgaris.
Conclusion:
The isolated compound (1R,3aR,5aR,5bR,7aR,9S,11aR,11bR,13aR,13bR)-3a,5a,5b,8,8,11ahexamethyl-
1-prop-1-en-2-yl-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13bhexadecahydrocyclopenta[
a]chrysen-9-ol showed good anti-bacterial and anti-fungal activity against
selected pathogens.
Keywords: Andrographis echioides, leaves, anti-bacterial, anti-fungal, Isolated compound
Publication date: 01/09/2020
https://ijbpas.com/pdf/2020/July/MS_IJBPAS_2020_50451.pdf
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https://doi.org/10.31032/IJBPAS/2020/9.7.5045
