ABSTRACT

Background: The present study emphasized that prevalence and molecular genetics of methicillin- resistant Staphylococcus aureus from clinical isolates associate with hospital associated disease. Materials and Methods: Staphylococcus aureus was isolated from different clinical samples such as urine, blood, sputum, pus/wound swab and cerebrospinal fluid (CSF). The clinical samples were collected from different pathology laboratories of Nagpur City. These samples were screened on different bacteriological media and identified on the basis of their morphological, cultural and biochemical characteristics and confirmed by 16s rRNA. Results: During this study, 1465 different clinical samples were tested in which 1255 (85.66%) samples showed growth of bacteria. Out of 1255 clinical samples, 1157 (92.19%) different strains of bacteria were isolated. Among the isolated organisms Staphylococcus aureus was found to be 201 (17.37%). For testing with getotyping of isolates by multiplex PCR detaction using mecA, vanA, OXA gene. Among the 201 isolates, 195 strains of S. aureus harbouring single mecA gene and the percentage was found to be 97.01%. 102 strains of S. aureus showed resistance to two genes - mecA + OXA gene was found to be 50.75% whereas 74 (36.82%) strains of S. aureus showed resistance to three genes - mecA + vanA + OXA gene. Conclusion: Identification of the genes is necessary for the surveillance of their transmission in hospitals. Keywords: Molecular genetics, MRSA, Multiplex PCR, mecA gene
Publication date: 01/09/2020

https://ijbpas.com/pdf/2020/September/MS_IJBPAS_2020_5180.pdf

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https://doi.org/10.31032/IJBPAS/2020/9.9.5180